Co-production of biohydrogen and biomethane utilizing halophytic biomass Atriplexcrassifolia by two-stage anaerobic fermentation process

The excessive use of fossil has resulted in the drastic exhaustion natural energy sources, leading to environmental challenges and crises. Owing rising demand there is a dire need shift towards renewable energies from lignocellulosic biomass. present study assessed co-production biohydrogen (H 2 ) biomethane (CH 4 by utilizing less explored halophyte Atriplexcrassifolia. Various reaction parameters were evaluated for their effect on production batch experiments. One parameter at time experimental strategy was chosen optimization. Hydrogen methane yields along with rates different incubation times, temperatures, pH, substrate concentrations, inoculum sizes acidogenesis methanogenesis stages, respectively. In first stage, maximum cumulative hydrogen 66 ± 0.02 mL, yield 13.2 0.03 mL/g, rate (HPR) 1.37 0.05 mL/h attained when mixture (5 g Atriplexcrassifolia 10 mL pretreated sewage sludge) processed 37°C pH 5.5 after 48 h incubation. While second production, i.e., 343 0.12 (MY) 8.5 0.07 mL/mL, (MPR) 0.8 achieved 18 days (40 hydrogenic slurry 80 inoculum) 45°C 8. Furthermore, 51% 24% rise respectively recorded gases produced these optimized conditions. results ensure as an imperative resource proposed that effective optimization process further increased coproduction biomethane.